Response of Size-fractionated Phytoplankton to Humic Acids in the Seawater of Yeongsan River Estuary

Sehee Kim; Yongsik Sin

doi:10.4217/OPR.2023011

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2023 Vol.45, Issue 3 Preview Page Next Page

Article

Response of Size-fractionated Phytoplankton to Humic Acids in the Seawater of Yeongsan River Estuary 영산강 하구에서 부식산이 식물플랑크톤에 미치는 영향

30 September 2023. pp. 113-123

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Abstract

Humic substances are refractory organic compounds that are relatively low in biological activity but known to stimulate phytoplankton growth in estuarine and marine environments. The effect of humic substances on phytoplankton growth was investigated in the seawater zone of the Yeongsan River estuary where an episodic anthropogenic freshwater is discharged, affecting its water properties directly. Water samples and data of water properties were collected at three stations (Sts. A–C) along the channel of the seawater zone in February, May, August and November, 2009. The collected water samples were incubated after humic acids (HA) were added in mesocosm experiments. Phytoplankton (chlorophyll-a) were fractionated into net- (> 20 µm) and nano-size (< 20 µm) to examine the response of phytoplankton according to size. Their response to HA treatment was assessed by repeated measures analysis of variance (RM- ANOVA). The experiments showed that phytoplankton biomass (chlorophyll-a) significantly increased after HA were added at the stations near the sea dike. Especially, nano-sized chlorophyll-a concentrations increased significantly throughout the seasons. This indicates that understanding the behavior of refractory organic matters such as humic substances is required to better manage altered estuarine ecosystems including the Yeongsan River estuary which are affected by episodic discharge of freshwater from sea dikes.

Keywords

Humic Acids

size-fractionated chlorophyll-a

phytoplankton

Yeongsan River estuary

Mesocosm experiment

References

윤보배, 이어진, 강태안, 신용식 (2013) 하구언 담수방류와 영산강 하구 식물플랑크톤 생체량 및 환경인자의 장기변동. 생태와 환경 46(2):205-214

이연정, 정병관, 신용식, 김성환, 신경훈 (2013) 탄소 및 질소 안정동위원소 비를 이용한 영산강하구역 유기물 기원 추정 연구. 생태와 환경 46(2):175-184

Adey WH, Loveland K (2007) The input of organic energy. Dynamic Aquaria. Academic Press, Amsterdam, pp 93-100 10.1016/B978-0-12-370641-6.50015-7

Aiken GR, Mcknight DM, Wershaw RL, Maccarthy P (1985) Humic substances in soil, sediment, and water. Soil Sci 142(5):323 10.1097/00010694-198611000-00011

Álvarez-Góngora C, Herrera-Silveira JA (2006) Variations of phytoplankton community structure related to water quality trends in a tropical karstic coastal zone. Mar Pollut Bull 52(1):48-60 10.1016/j.marpolbul.2005.08.00616194550

Canuel EA, Hardison AK (2016) Sources, ages, and alteration of organic matter in estuaries. Annu Rev Mar Sci 8:409-434 10.1146/annurev-marine-122414-03405826407145

Carlsson P, Granéli E (1993) Availability of humic bound nitrogen for coastal phytoplankton. Estuar Coast Shelf Sci 36(5):433-447 10.1006/ecss.1993.1026

Carlsson P, Granéli E, Segatto AZ (1999) Cycling of biologically available nitrogen in riverine humic substances between marine bacteria, a heterotrophic nanoflagellate and a photosynthetic dinoflagellate. Aquat Microb Ecol 18:23-36 10.3354/ame018023

Duan S, Bianchi TS (2006) Seasonal changes in the abundance and composition of plant pigments in particulate organic carbon in the lower Mississippi and Pearl Rivers. Estuaries Coast 29(3):427-442 10.1007/BF02784991

Eljarrat E (2012) Methodologies for sample preservation and stabilization. In: Pawliszyn J (ed) Comprehensive sampling and sample preparation. Elsevier, Barcelona, pp 31-49 10.1016/B978-0-12-381373-2.00004-1

Filella M, Buffle J, Parthasarathy N (2005) Humic and fulvic compounds. In: Worsfold P, Townshend A, Poole C (eds) Encyclopedia of analytical science. Elsevier, Geneva, pp 288-298 10.1016/B0-12-369397-7/00260-0

Francko DA (1986) Epilimnetic phosphorus cycling: influence of humic materials and iron on coexisting major mechanisms. Can J Fish Aquat Sci 43:302-310 10.1139/f86-039

Froneman PW, Pakhomov EA, Balarin MG (2004) Size- fractionated phytoplankton biomass, production and biogenic carbon flux in the eastern Atlantic sector of the Southern Ocean in late austral summer 1997-1998. Deep- Sea Res 51(22-24):2715-2729 10.1016/j.dsr2.2002.09.001

Hedges JI, Keil RG (1999) Organic geochemical perspectives on estuarine processes: sorption reactions and consequences. Mar Chem 65(1-2):55-65 10.1016/S0304-4203(99)00010-9

Hein M, Pedersen MF, Sand-Jensen K (1995) Size-dependent nitrogen uptake in micro- and macroalgae. Mar Ecol-Prog Ser 118:247-253 10.3354/meps118247

Jouenne F, Lefebvre S, Véron B, Lagadeuc Y (2005) Biological and physicochemical factors controlling short-term variability in phytoplankton primary production and photosynthetic para meters in a macrotidal ecosystem (eastern English Channel). Estuar Coast Shelf S 65(3):421-439 10.1016/j.ecss.2005.05.023

Lee J, Park J, Shin Y, Lee B, Chang N, Cho J, Kim S (2009) Effect of dissolved organic matter on the growth of algae, Pseudokirchneriella subcapitata, in Korean lakes: the importance of complexation reactions. Ecotoxicol Environ Saf 72(2):335-343 10.1016/j.ecoenv.2008.01.01318313752

Lipczynska-Kochany E (2018) Humic substances, their microbial interactions and effects on biological transformations of organic pollutants in water and soil: a review. Chemosphere 202:420-437 10.1016/j.chemosphere.2018.03.10429579677

McLusky DS, Elliot M (2004) The estuarine ecology: ecology, threats and management. Oxford University Press, New York, 241 p

Middelburg JJ, Herman PM (2007) Organic matter processing in tidal estuaries. Mar Chem 106:127-147 10.1016/j.marchem.2006.02.007

Packham RF (1964) Studies of organic color in natural water. Proc Soc Water Treat Exam 13:316-334

Prakash A, Rashid MA (1968) Influence of humic substances on the growth of marine phytoplankton: Dinoflagellates. Limnol Oceanogr 13(4):598-606 10.4319/lo.1968.13.4.0598

Prakash A, Rashid MA, Jensen A, Subha Rao DV (1973) Influence of humic substances on the growth of marine phytoplankton: diatoms. Limnol Oceanogr 18(4):516-524 10.4319/lo.1973.18.4.0516

Ryther JH (1969) Photosynthesis and fish production in the sea: the production of organic matter and its conversion to higher forms of life vary throughout the world ocean. Science 166(3901):72-76 10.1126/science.166.3901.725817762

See JH, Bronk DA, Lewitus AJ (2006) Uptake of Spartina‐derived humic nitrogen by estuarine phytoplankton in nonaxenic and axenic culture. Limnol Oceanogr 51(5):2290-2299 10.4319/lo.2006.51.5.2290

Sin Y, Hyun B, Jeong B, Soh H (2013) Impacts of eutrophic freshwater inputs on water quality and phytoplankton size structure in a temperate estuary altered by a sea dike. Mar Environ Res 85:54-63 10.1016/j.marenvres.2013.01.00123410639

Sin Y, Jeong B (2015) Short-term variations of phytoplankton communities in response to anthropogenic stressors in a highly altered temperate estuary. Estuar Coast Shelf Sci 156:83-91 10.1016/j.ecss.2014.09.022

Sin Y, Kim S (2022) Effects of humic acids on size and species composition of phytoplankton in a eutrophic temperate estuary. Appl Sci 12(20):10223 10.3390/app122010223

Sin Y, Lee E, Lee Y, Shin K (2015) The river-estuarine continuum of nutrients and phytoplankton communities in an estuary physically divided by a sea dike. Estuar Coast Shelf Sci 163:279-289 10.1016/j.ecss.2014.12.028

Sin Y, Lee H (2020) Changes in hydrology, water quality, and algal blooms in a freshwater system impounded with engineered structures in a temperate monsoon river estuary. J Hydrol Reg Stud 32:100744 10.1016/j.ejrh.2020.100744

Steinberg CEW (2003) Ecology of humic substances in freshwaters: determinants from geochemistry to ecological niches. Springer, Berlin, 440 p 10.1007/978-3-662-06815-1

Stuermer DH, Harvey GR (1977) The isolation of humic substances and alcohol-soluble organic matter from seawater. Deep-Sea Res 24(3):303-309 10.1016/S0146-6291(77)80010-6

Thurman EM (1985) Organic geochemistry of natural waters. Springer, Dordrecht, 497 p 10.1007/978-94-009-5095-5PMC1144773

Totti C, Cangini M, Ferrari C, Kraus R, Pompei M, Pugnetti A, Socal G (2005) Phytoplankton size-distribution and community structure in relation to mucilage occurrence in the northern Adriatic Sea. Sci Total Environ 353:204-217 10.1016/j.scitotenv.2005.09.02816213005

Tranvik LJ, von Wachenfeldt E (2009) Interactions of dissolved organic matter and humic substances. In: Likens EG (ed) Encyclopedia of inland waters. Academic Press, Uppsala, pp 754-760 10.1016/B978-012370626-3.00109-5

Yoon B, Jung Y, Sin Y (2023) Assessing nutrient limitation in Yeongsan River estuary using bioassay experiments. J Mar Sci Eng 11(7):1337 10.3390/jmse11071337

국문 참고자료의 영문표기 English translation / Romanization of references originally written in Korean

Yoon BB, Lee EJ, Kang TA, Shin YS (2013) Long-term change of phytoplankton biomass (chlorophyll-a), environmental factors and freshwater discharge in Youngsan estuary. KJEE - Korean J Ecol Environ 46(2):205-214 10.11614/KSL.2013.46.2.205

Lee YJ, Jeong BK, Shin YS, Kim SH, Shin KH (2013) Determination of the origin of particulate organic matter at the estuary of Youngsan River using stable isotope ratios (δ13C, δ15N). Korean J Ecol Environ 46(2):175-184 10.11614/KSL.2013.46.2.175

Information

Publisher :Korea Institute of Ocean Science and Technology
Publisher(Ko) :한국해양과학기술원
Journal Title :Ocean and Polar Research
Journal Title(Ko) :Ocean and Polar Research
Volume : 45
No :3
Pages :113-123
Received Date : 2023-07-24
Revised Date : 2023-09-06
Accepted Date : 2023-09-06
DOI :https://doi.org/10.4217/OPR.2023011

Ocean and Polar Research ISSN:2234-7313(Online) Ocean and Polar Research

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국문 참고자료의 영문표기 English translation / Romanization of references originally written in Korean