Antioxidant and Anti-inflammatory Activities of the Halophyte Cyrtomium falcatum

Hyunmo Kim; Hojun Kim; Chang-Suk Kong; Bong Ho Lee; Hyun-Bo Sim; Youngwan Seo

doi:10.4217/OPR.2021.43.3.113

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Article

Antioxidant and Anti-inflammatory Activities of the Halophyte Cyrtomium falcatum 염생식물 도깨비고비의 항산화 및 항염증 효과

30 September 2021. pp. 113-126

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Abstract

In the present study, the halophyte C. falcatum extract and its solvent fractions (n-hexane, 85% aqueous methanol, n-butanol, and water) were evaluated for antioxidant and anti-inflammatory activities. Antioxidative ability was measured by DPPH radical, intracellular reactive oxygen species (ROS) and peroxynitrite scavenging, DNA oxidation inhibition, and ferric reducing antioxidant power (FRAP). For DPPH radical and peroxynitrite scavenging, DNA oxidation inhibition, and FRAP, 85% aq.MeOH and n-BuOH fractions showed significant scavenging activity. For production of intracellular ROS in HT-1080 cells, 85% aq.MeOH fraction showed the highest scavenging activity. In addition, anti-inflammatory activity was also assessed by measuring the inhibitory effect against mRNA expression of pro-inflammatory factors (NO, IL-1β, IL-6 and COX-2) in LPS-stimulated Raw 264.7 macrophages. For NO production, crude extract exhibited a strong inhibitory effect at a concentration of 100 μg/ml. For mRNA expression of pro-inflammatory cytokines (IL-1β, IL-6, and COX-2), n-BuOH greatly suppressed expression levels of IL-1β and IL-6 at 100 μg/ml concentration while 85% aq. MeOH fraction significantly inhibited that of COX-2 even at 100 μg/ml. These results suggest that C. falcatum may be used as a potential source for the development of a natural antioxidant or anti-inflammatory agent.

Keywords

Cyrtomium falcatum

halophyte

reactive oxygen species (ROS)

antioxidant

anti-inflammatory

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Information

Publisher :Korea Institute of Ocean Science and Technology
Publisher(Ko) :한국해양과학기술원
Journal Title :Ocean and Polar Research
Journal Title(Ko) :Ocean and Polar Research
Volume : 43
No :3
Pages :113-126
Received Date : 2021-06-10
Revised Date : 2021-07-02
Accepted Date : 2021-07-06
DOI :https://doi.org/10.4217/OPR.2021.43.3.113

Ocean and Polar ResearchISSN:2234-7313(Online)한국해양과학기술원

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국문 참고자료의 영문표기 English translation / Romanization of references originally written in Korean