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2022 Vol.44, Issue 4 Preview Page

Article

Effects of Ovarian Parasite Marteilioides chungmuensis on the Reproduction of the Pacific Oyster Crassostrea gigas Assessed by Histology and Enzyme-Linked Immunosorbent Assay (ELISA)
Yanin Limpanont1
,
Hyun-Sil Kang2
,
Young-Ghan Cho3
,
Jong-Seop Shin3
,
Nobuhisa Kajino3
,
Jeong-Hwa Kim3
,
Hyun-Ki Hong3
,
Kwang-Sik Choi3*
1Department of Social and Environmental Medicine, Faculty of Tropical Medicine, Mahidol University, Bangkok 10400, Thailand
2Tidal Flat Research Center, National Institute of Fisheries and Science, Gunsan 54014, Korea
3Department of Marine Life Science and Marine Science Institue (BK21 FOUR), Jeju National University, Jeju 63243, Korea
*Corresponding Author
30 December 2022. pp. 319-329
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Abstract

The paramyxean parasite Marteilioides chungmuensis infects the cytoplasm of the eggs of Pacific oysters Crassostrea gigas, resulting in spawning failure of the infected females. Such infected eggs appear as bump-like nodules on the body in late fall when most of the uninfected females complete spawning. In this study, we estimated the quantity of the infected eggs using an enzyme-linked immunosorbent assay (ELISA), which is destroyed by M. chungmuensis parasitism. In December, the infected oysters collected from Tongyoung on the south coast exhibited numerous yellowish bump-like nodules as signs of infection. In histology, the infected oysters exhibited mature eggs in the follicle, which were heavily infiltrated by hemocytes. ELISA indicated that the infected egg mass accounted for 7.52±5.50 percent of the body weight, suggesting the ovarian parasite causes substantial reproductive loss. Histology also indicated that the infected oysters are in a poor nutritional condition, as the digestive gland atrophy (DGA) level is comparatively higher than the uninfected oyster. The total carbohydrate contents in the infected oysters (108.68±44.41 mg/g dry wt) were significantly lower than in uninfected oysters (269.76±50.97 mg/g dry wt), suggesting that M. chungmuensis parasitism also affected the energy storage capacity of the host during the resting stage.

Keywords
Marteilioides chungmuensis
ovarian oyster parasite, reproductive effort
Crassostrea gigas
ELISA
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Information
  • Publisher :Korea Institute of Ocean Science and Technology
  • Publisher(Ko) :한국해양과학기술원
  • Journal Title :Ocean and Polar Research
  • Journal Title(Ko) :Ocean and Polar Research
  • Volume : 44
  • No :4
  • Pages :319-329
  • Received Date : 2022-09-14
  • Revised Date : 2022-10-17
  • Accepted Date : 2022-10-18
  • DOI :https://doi.org/10.4217/OPR.2022023
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