Limonium Tetragonum Enhances Osteoblastogenesiswhile Suppressing the Adipocyte Differentiation

Jung-Ae Kim; Byul-Nim Ahn; Jung Hwan Oh; Fatih Karadeniz; Jung Im Lee; Youngwan Seo; Chang-Suk Kong

doi:10.4217/OPR.2022002

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2022 Vol.44, Issue 1 Preview Page Next Page

Article

Limonium Tetragonum Enhances Osteoblastogenesiswhile Suppressing the Adipocyte Differentiation

30 March 2022. pp. 29-38

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Abstract

Halophytes are plants that live in harsh environments in coastal regions and are known for their diverse chemical compositions. Limonium tetragonum, a halophyte endemic to Korean shores, is known for its bioactive compounds and is utilized in folk medicine. In this study L. tetragonum extract (LHE) was used to determine and evaluate its anti-osteoporotic properties. Pre-adipocyte and pre-osteoblasts were induced to differentiate along with LHE treatment, and their differentiation was evaluated using differentiation markers. LHE treatment decreased lipid accumulation in 3T3-L1 preadipocytes during adipogenesis. Results indicated that the LHE treatment also decreased the levels of key adipogenic transcription factors: PPARγ, SREBP1c, and C/EBPα. Enhancing osteoblastogenesis by LHE treatment was confirmed in osteoblastogenesis-induced MC3T3-E1 pre-osteoblasts. Cells treated with LHE resulted in increased calcification and alkaline phosphatase (ALP) activity compared with osteoblasts without LHE treatment. Pro-osteogenic and anti- adipogenic effects were also confirmed in D1 murine mesenchymal stromal cells which are capable of differentiation into both adipocytes and osteoblasts. LHE hindered adipogenesis and enhanced osteoblastogenesis in D1 MSCs in a similar fashion. In conclusion, L. tetragonum is believed to possess the potential to be utilized as a nutraceutical ingredient against osteoporotic conditions.

Keywords

3T3-L1

Limonium tetragonum

MC3T3-E1

mesenchymal stem cell

osteoporosis

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Information

Publisher :Korea Institute of Ocean Science and Technology
Publisher(Ko) :한국해양과학기술원
Journal Title :Ocean and Polar Research
Journal Title(Ko) :Ocean and Polar Research
Volume : 44
No :1
Pages :29-38
Received Date : 2021-12-01
Revised Date : 2021-12-30
Accepted Date : 2022-01-09
DOI :https://doi.org/10.4217/OPR.2022002

Ocean and Polar Research ISSN:2234-7313(Online) Ocean and Polar Research

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