Morphological and molecular investigation of Melosira octogona Schmidt (Melosirales,  Bacillariophyta) isolated from the Jeju Island

Sung Min An; Kichul Cho; Grace Choi

doi:10.4217/OPR.2024010

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2024 Vol.46, Issue 2 Preview Page

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Morphological and molecular investigation of Melosira octogona Schmidt (Melosirales, Bacillariophyta) isolated from the Jeju Island

30 June 2024. pp. 123-129

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Abstract

Melosira octogona Schmidt has been documented primarily through illustrations and light microscopy photographs, resulting in incomplete morphological descriptions. In this study, the morphological characteristics of M. octogona were investigated using scanning electron microscopy, and the small subunit rDNA of the strain isolated from the coast of Jeju Island, South Korea, was analyzed for the first time. The cells were organized in short chains, with the frustules taking on an octagonal shape in the girdle view because of the straight, obliquely oriented valve margin. The cells were joined together in specific pairs by a cingulum. Imaging analysis via SEM revealed the presence of a hemispherical valve with a well-developed corona on the valve face, along with stellate thickening and rimoportulae. The epicingulum was composed of six ligulate bands punctuated by rows of circular to elongated pores. Genetic analysis of the 18S rDNA sequence suggested a close relationship between M. octogona and Melosira sp. (KJ961704), demonstrating a high degree of similarity. Meanwhile, it exhibited comparatively low similarity (99.0 %) to Melosira cf. octogona (AY485518). The results of our analysis can enhance the precise recognition of M. octogona and enrich our understanding of its phylogenetic position within the order Melosirales.

Keywords

diatom

Jeju Island

Melosira

scanning electron microscopy

SSU sequence

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Information

Publisher :Korea Institute of Ocean Science and Technology
Publisher(Ko) :한국해양과학기술원
Journal Title :Ocean and Polar Research
Journal Title(Ko) :Ocean and Polar Research
Volume : 46
No :2
Pages :123-129
Received Date : 2024-03-22
Revised Date : 2024-05-29
Accepted Date : 2024-06-05
DOI :https://doi.org/10.4217/OPR.2024010

Ocean and Polar Research ISSN:2234-7313(Online) Ocean and Polar Research

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