Inhibition of Lipase Activity and Preadipocyte Differentiation in3T3-L1 Cells Treated with Sargassum horneri Extract

Ji Woo Hong; Ha Young Park; Jae Hyun Park; So Hee Kim; Han A Kim; Jin-Woo Kim

doi:10.4217/OPR.2022006

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2022 Vol.44, Issue 1 Preview Page Next Page

Article

Inhibition of Lipase Activity and Preadipocyte Differentiation in3T3-L1 Cells Treated with Sargassum horneri Extract 괭생이모자반 추출물의 리파아제 저해 활성 및 3T3-L1 지방전구세포 분화억제 효과

30 March 2022. pp. 61-67

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Abstract

In this study, in order to evaluate the anti-obesity effect of sargassum horneri extract, the effects of the extract on lipase activity and preadipocyte differentiation in 3T3-L1 cells were investigated. S. horneri extract between 0.0 and 1.0 mg/mL showed no cytotoxicity and inhibited lipase activity by 68.1%. When S. horneri extract was utilized at levels of 0.25, 0.5, and 1.0 mg/mL in 3T3-L1 cells, preadipocytes differentiation decreased by 11.4, 19.7, and 25.6%, respectively, showing anti-obesity effects. In addition, after treatment with 1.0 mg/mL S. horneri extract, the mRNA expression levels of sterol regulatory element binding proteins-1c (SREBP-1c), peroxisome proliferator activated receptor-γ (PPAR-γ), CCAAT enhancer binding protein-α (CEBP-α), fatty acid synthase (FAS), and stearoyl-CoA desaturase1 (SCD1) in 3T3-L1 cells were significantly decreased (p < 0.05) by 65.2, 54.9, 50.0, 33.8, and 33.8% respectively. These results showed that S. horneri extract suppresses lipase activity and prophylactic preadipocyte differentiation in 3T3-L1, and thus can be used as an anti-obesity agent in functional foods and medicines.

Keywords

sargassum horneri

marine species

adipogenesis

3T3-L1

anti-obesity

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Information

Publisher :Korea Institute of Ocean Science and Technology
Publisher(Ko) :한국해양과학기술원
Journal Title :Ocean and Polar Research
Journal Title(Ko) :Ocean and Polar Research
Volume : 44
No :1
Pages :61-67
Received Date : 2022-01-04
Revised Date : 2022-01-20
Accepted Date : 2022-02-25
DOI :https://doi.org/10.4217/OPR.2022006

Ocean and Polar Research ISSN:2234-7313(Online) Ocean and Polar Research

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